Lifetime Upgrade Kit for Leica TCS SP2
Fluorescence lifetime imaging with ps resolution
Ultra-high sensitivity due to TCSPC technique
Zoom and image rotation functions of TCS SP2 applicable
FRET experiments
Fluorescence quenching
Autofluorescence
Separation of chromophores by lifetime
The setup employs an advanced TCSPC imaging technique featuring both high count rate and low differential nonlinearity. It contains the usual building blocks (CFDs, TAC, ADC) in the ‘reversed startstop’ configuration together with a scanning interface and a large histogram memory integrated on one PC board. For each photon the TCSPC module determines the time within the fluorescence decay function and the location within the scanning area. These values are used to address a histogram memory in which the events are accumulated. Thus, in the memory the distribution of the photon density over X, Y, and the time within the fluorescence decay function builds up. The result can be interpreted as a two dimensional (X, Y) array of fluorescence decay curves or as a sequence of fluorescence images for different times (t) after the excitation pulse.
Requirements to SP2 Microscope
Ti:Sa Laser installed
Non-descanned output installed 1)
FLIM Upgrade Kit - Basis Version
Detector Options
Part List
Detector
PMH-100-0
H7422-40
R3809U-52
Attached to
NDD Output1)
NDD Output 1)
NDD Output 1)
Preamplifier
none
HFAC-26-1
HFAC-26-01
Controller
DCC-100 2)
DCC-100
DCC-100
HV Power Supply
none
none
FuG HCN-14-3500A
Adapter
NDD Adapter Assembly
Data Analysis
SPCImage data analysis software
1) bh will grant no warranty for detectors damaged by exposure to roomlight or light from halogene or mercury lamp
2) Recommended
描述 [描述] Ultra-high sensitivity due to TCSPC technique