STEDYCON
a class of its own
The STEDYCON upgrades your existing widefield system to a confocal microscope and STED nanoscope with a resolution down to 30 nm. All that’s required is a free camera port and a good objective lens. With its super-intuitive user interface, the STEDYCON provides an intelligent microscope platform that enables everyone to acquire superb superresolution images after only minutes of training. Define your experiment and enjoy fine imaging at the push of a button!
1um
Confocal
STED
Description
Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to Abberior STAR 580 and Abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.
Description
Confocal Imaging of a large sample region. Shown is a 2.8 mm x 2.5 mm region, acquired using 35 by 29 tiles using the STEADYFOCUS and stitched with SVI Huygens. Sample: mammalian cells immunolabelled for the mitochondrial protein TOM20 (STAR RED, red), double-stranded DNA to visualize mitochondrial DNA (STAR ORANGE, green), Phalloidin to label F-actin (STAR GREEN, gray), and DAPI to label nuclei. Sample preparation by Abberior GmbH.
1um
Confocal
STED
Description
Immunolabelling of βIV-spectrin, a scaffolding protein of the axon initial segment, using a self-made primary antibody and Alexa594 secondary antibody. Cryo-section of mouse neocortex cut at 20 µm. Images were acquired using a STEDYCON attached to a Zeiss AxioImager Z2. The sample was kindly provided by Dr. Maren Engelhardt, Institute of Neuroanatomy, Medical Faculty Mannheim, Heidelberg University.
STEDYCON
super small and super stable
The incredibly compact STEDYCON has the size of a shoebox, yet it is a full-fledged confocal and STED microscope. It fits on every microscope frame with a camera port. Get in touch with us to upgrade your system!
The STEDYCON is service and maintenance-free: The laser beams are aligned by design. So, there is no need to align the system, ever, and it works reliably every day.
STEDYCON
extremely user-friendly
Save your time with the STEDYCON software: only minutes of training are required to acquire STED and confocal images. Thanks to the powerful dye database behind, only minimal user input is needed, and even with unkown samples, you get with 3 clicks to the first STED image!
Our software is browser-based, so run it from any device, from any platform, from anywhere!
STEADYFOCUS
in focus, forever
The STEADYFOCUS laser runs side-by side with the imaging
beam path. No dichroics are required and there is absolutely no
interference with imaging.
The autofocus laser is coupled into the beam path using a dichroic mirror,
which interferes with imaging.
Our STEADYFOCUS keeps the focus position stable. Forever. During
STED imaging, when browsing through the sample, or for hours of
time-lapse imaging, there is no need for refocussing, the STEADYFOCUS
does the work for you. And everything you need for it is integrated inside
the STEDYCON head, so it works on upright microscopes, too!
STEADYFOCUS
tiling and stitching is a piece of cake
Discover your samples! Acquire a quick tiled overview as a basis for tiled scans with overlap or for multi-position imaging. Define you experiment, let the microscope image for you, with active drift correction thanks to our STEADYFOCUS!
After tiled image acquisition, send over the data to Huygens, for easy, fast and reliable stitching – all needed parameters are automatically transferred.
Multipositioning
let our STEDYCON do the work
Discover your whole sample, and then take your time to define your experiment – at which position would you like to acquire a z-stack, or a single image, or a tiled scan? Then let the microscope do the imaging for you. Increase your working efficiency, let it run over night, or already prepare your next experiment! It saves time and endless clicks …
SVI Huygens
deconvolution and analysis
Transfer images and metadata from the STEDYCON to Huygens with a single click. Start deconvolution in an instant, with our calibrated PSF already included. At the same time, use the incredibly powerful Huygens analysis and 3D visualization platform, or use the Huygens movie maker for all images taken with the STEDYCON.
STEDYCON to work in 1:28 min
Watch how the STEDYCON is unpacked, attached to the microscope frame, and used to immediately acquire excellent super-resolution images – no alignment needed, and just enough time to cook a coffee.
STEDYCON @ work
The STEDYCON upgrades any microscope frame, as long as a free camera port is available, and a good objective lens is key. All lasers, scanners, and detectors are already inside our STEDYCON box. The STEDYCON is super flexible and extremely stable, as it works reliably not only in climatized labs, but also on conferences, and even in our living rooms!
Reliable, automatized, and easy-to-use, this is how to work with your STED microscope!
super-easy to use
Only minutes of training for new users
Powerful dye database for minimal user input
Multiposition imaging to let the microscope work for you