EASY3D
super easy, super resolution
3D super-resolution microscopy has never been easier. The patented single-beam light path of our EASY3D module allows you to capture the complete three dimensional structure of your sample features. Furthermore, EASY3D is compatible with any moderate- to high-NA objective lens and can correct aberrations in the STED beam, giving you full freedom to embed and image your sample in the best possible way.
Description
Movie through a 2-color EASY3D RESCue STED volume stack of a
cleared adult kidney sample of a rat. Shown is a movie of an EASY3D
and RESCUE volume stack of a renal corpuscle showing Nephrin (red,
Abberior STAR635P) structures inbetween the Podocin slits (green,
AlexaFluor594).
Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH
Stockholm, Sweden.
Expert Devices:
EASY3D
Description
Surface Rendering of 2-color EASY3D volume of a cleared adult
kidney sample of a rat. Shown is a reconstruction of an EASY3D
volume stack of a renal corpuscle showing Nephrin (red, Abberior
STAR635P) structures inbetween the Podocin slits (green,
AlexaFluor594).
Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH
Stockholm, Sweden.
Expert Devices:
EASY3D
Description
Nuclear pore complexes (NUP) and tubulin imaged with confocal and
EASY3D-STED microscopy.
Expert Devices:
EASY3D
Description
Tubulin imaged with EASY3D-STED vs confocal microscopy.
Expert Devices:
EASY3D
Description
Two-color EASY3D-STED image of tubulin and giantin versus its
confocal low-resolution counterpart.
Expert Devices:
EASY3D
Description
Mouse inner ear hair cells imaged with superresolution in all three
dimensions using EASY3D. Animation was generated directly from
raw data. Actin stain (Abberior STAR 580 Phalloidin, blue) and
Whirlin immunostain (Abberior STAR 635P secondaries, yellow-red).
Scalebar 1 µm.
Samples were prepared by Dr. Christian Vogl, InnerEarLab, UMG
Göttingen, Germany
Expert Devices:
EASY3D
EASY3D
bright images due to optical abberation control
Conventional 3-dimensional STED <
is often impared by aberrations caused by refractive index (RI) mismatches – reducing brightness and resolution with increasing sample depth.
> EASY3D STED with adaptive optics
corrects optical aberrations – allowing bright 3D-superresolution images at much greater depth.
EASY3D enables deep imaging of cleared adult kidney samples. Comparison of a XZ-slice deep into a renal corpuscle using conventional 3D STED imaging and EASY3D imaging. EASY3D allowed imaging up to 80 µm into cleared rat kidney tissue using an oil objective. Without correction of aberrations in the STED beam, the mismatch between immersion oil and sample leads to complete signal loss. Labels: Nephrin (red, Abberior STAR635P) and Podocin (green, AlexaFluor594).
Sample was prepared by D. Unnersjö Jess and H.G. Blom @ KTH Stockholm, Sweden.
EASY3D
why do we call it “easy”
Tune the resolution in XY and Z to fit your imaging problem
Use any combination of 2D or 3D STED phase plates or any user-defined phase modulation
Aberration correction: keep the STED PSF in shape even deep inside your sample (> 20 μm)
Switch between objective lenses on the fly: oil immersion, water immersion, silicone oil immersion…
Resolution EASY3D: 90 × 90 × 90 nm minimum, 75 × 75 × 75 nm typical
Resolution 2D: better than 30 × 30 nm
Any abberior MINFLUX, INFINITY and FACILITY system can be equiped with the EASY3D device
The abberior EASY3D device uses a programmable spatial light modulator (SLM) to create phase patterns required for 2D and 3D STED microscopy. At the same time, it can also be used to correct for optical aberrations of the STED beam.
The SLM allows a revolutionary design with only one STED beam instead of two separate STED beams for lateral (XY) and axial (Z) resolution enhancement, respectively. The resolution enhancement can be tuned between pure XY and mainly Z enhancement. The single-beam design obsoletes any beam recombination and alignment resulting in outstanding system stability.
Aberrations do matter! Each and every day. This measurement impressively demonstrates the effect of refractive index mismatch. When an oil immersion lens is used to image a layer of fluorescent beads imaged through 13 μm of water, the image suffers from a significant loss in fluorescence intensity. This results in unusable images, both for confocal and for STED imaging.
In contrast, when a water immersion lens is used, both layers of beads are imaged with the same quality The abberior EASY3D design allows you to change the objective lens on the fly and to use the most suitable lens in STED mode also!
Additionally, you can further correct aberrations in all beams with our ADAPTIVE OPTICS device!
A aberration correction with our programmable spatial light modulator (SLM) allows you to image with 3D STED resolution down to 180 μm into the sample! This is only possible if you can correct the Z-STED zero depending on the sample depth.
3D, the easy way
Tune the resolution in xy and z to fit your imaging problem
Switch between objective lenses on the fly: oil immersion, water immersion, silicone oil …
Resolution 3D: 90 × 90 × 90 nm minimum, 75 × 75 × 75 nm typical
Resolution 2D: better than 30 × 30 nm
描述 [描述] EASY3D module allows you to capture the complete three dimensional structure of your sample features.