FLIM – fluorescence lifetime imaging
adding the 5th dimension
At abberior, we not only work on increasing the amount of spatial resolution information you get from your samples. We’ve integrated lifetime imaging into our systems, to give you the best data not only in space, but also in time. Because at abberior, every dimension counts.
5um
Confocal FLIM
STED FLIM
Description
Confocal FLIM and STED FLIM image of mammalian cells labeled with antibodies against lamin/ Atto647N and DNA/ abberior STAR 635P.
Expert Devices:
FLIM
5um
Confocal FLIM
STED FLIM
Description
FLIM image of mammalian cells labeled with antibodies against Tom20/Atto 647N and Nup153/abberior STAR 635P. Note that nuclear pore (Nup) complex subunits appear to be localized in the cytoplasm, which is due to the Nup import pathway into the nuclear membrane.
5um
Confocal
STED FLIM
Description
FLIM image of tubulin labeled with Atto647N and vimentin labeled with abberior STAR 635P. The two labels (Atto647N and abberior STAR 635P) have the same excitation spectra, but different lifetimes. Tubulin (red, short lifetime) and vimentin (green, long lifetime) can successfully be separated in the STED FLIM recording via their lifetimes.
FLIM
it’s high time to add it
All abberior microscopes, including the STEDYCON, can be equipped with fully integrated time-correlated single-photon counting (TCSPC) electronics for lifetime imaging, based on Becker & Hickl or PicoQuant hardware.
This way, lifetime data can be recorded simultaneously with standard intensity measurements, and can be written to disk for later analysis, or displayed as lifetime histograms or color-coded lifetime value. As is typical for abberior, raw data is always kept. For example, you can export an image where local lifetime is displayed in pseudo-colors, and you can inspect and save a lifetime stack, which e.g. for a 2D image carries the complete lifetime histogram in a third dimension.
FLIM
precise timing of life
Lifetime imaging with our pulsed lasers and TCSPC electronics gives you the possibility to sense the chemical environment and adds an extra dimension to your image data. You can use FLIM to gain local information about pH or the concentration of dyes for another type of contrast next to labeling with different dyes.
FLIM
clear-cut separation of dyes with spectral overlap
FLIM allows to separate fluorescence dyes by their lifetime, even when they fluoresce at the same wavelength. This means that STED and confocal imaging get additional channels!
Fluorescence lifetime imaging adds an extra layer of information
Confocal and STED FLIM imaging
Online display of STED FLIM images during the measurement
Online lifetime calculation and/or dye separation based on lifetime data
Full software integration with live evaluation of data
描述 [描述] not only work on increasing the amount of spatial resolution information you get from your samples