High-End Performance TCSPC Module
Time Channel Width Down to 813 fs
Electrical Time Resolution 2.5 ps RMS (6.6 ps FWHM)
Extremely Good Timing Stability
10 MHz Saturated Count Rate
Single Mode, Oscilloscope Mode, FLIM, PLIM, FLITS, FCS, Mosaic FLIM
Fluorescence Decay Recording
Phosphorescence Decay Recording
Fluorescence and Phosphorescence Lifetime Imaging
Photon Correlation
Single-Molecule Spectroscopy
Free Instrument Software for Windows 7/8/10 (Realtime Calculation and Fitting of FCS Curves)
Parallel Operation of Up to 4 Modules
Available as Multi-Module Package e.g. SPC-152N, SPC-153N and SPC-154N
The SPC-150N is a high-end performance at reasonable costs time-correlated single photon counting (TCSPC) imaging module with high-time resolution. The board can be used for traditional fluorescence lifetime measurements, single molecule spectroscopy, FCS and FCCS recording, FLIM, Mosaic FLIM, FLITS, and combined FLIM / PLIM applications. This module is also the basis of FLIM upgrades for various scanning microscopes. The board offers a PCI interface and can be easily installed in nearly all PCs.
General Information
The SPC-150N boards have a large internal memory and a fast bus interface and ultra-fast discriminators in the detector- and synchronization inputs. This allows the modules to obtain superior time resolution with detectors that both have low transit time spread and an extremely fast single photon response. They are recommended especially for superconducting detectors, MCP PMTs, and fast hybrid detectors with bi-alkali or multi-alkali cathodes. They offer all standard photon distribution modes, the continuous-flow mode, the FIFO (time-tag) mode and the FIFO Imaging (FLIM) mode is implemented. The SPC-150N board can thus be used for traditional fluorescence lifetime experiments, diffuse optical tomography, stopped flow experiments, single molecule spectroscopy, FCS and FCCS recording, FLIM, Mosaic FLIM, FLITS, and combined FLIM / PLIM. One or more SPC-150N modules are used for the bh DCS-120 confocal FLIM system. They are also the basis of standard FLIM systems for the Zeiss LSM 710 and 510 microscopes and various other confocal and multiphoton laser scanning microscopes.
Measurement Software Included
The SPCM operating and measurement software is included with all SPC series modules. SPCM provides online calculation and display (2D, 3D) of data (decay curves, FLIM, FCCS) acquired in multiple operation modes. SPCM software undergoes active continuous development. SPCM receives frequent updates with new features and bug fixes.
FLIM and FCS Data Analysis
For advanced fluorescence lifetime imaging (FLIM) and single-curve data analysis, please use SPCImage.
For advanced fluorescence correlation spectroscopy (FCS) and cross-correlation (FCCS) data analysis, please use Burst Analyzer.Custom Programming Libraries (DLL, LabVIEW)
For automation and custom software integration, DLLs and LabVIEW drivers are available.
Photon Channel
Principle
Constant Fraction Discriminator (CFD)
Discriminator Input Bandwidth
4 GHz
Time Resolution (FWHM/RMS, electr.)
6.6 ps / 2.5 ps
Variance in Time of IRF max.
< 1 ps over 50 s
Optimum Input Voltage Range
-30 mV to -500 mV
Min. Input Pulse Width
200 ps
Threshold
0 to -250 mV
Zero Cross Adjust
-100 mV to 100 mV
Synchronisation Channel
Principle
Constant Fraction Discriminator (CFD)
Discriminator Input Bandwidth
4 GHz
Optimum Input Voltage Range
-30 mV to -500 mV
Min. Input Pulse Width
200 ps
Threshold
0 to -250 mV
Frequency Range
0 to 150 MHz
Frequency Divider
1, 2, 4
Zero Cross Adjust
-100 mV to 100 mV
Time-to-Amplitude Converters / ADCs
Principle
Ramp Generator / Biased Amplifier
TAC Range
50 ns to 5 µs
Biased Amplifier Gain
1 to 15
Biased Amplifier Offset
0 % to 100 % of TAC Range
Time Range incl. Biased Amplifier
3.3 ns to 5 µs
Min. Time Channel Width
813 fs
ADC Principle
50 ns Flash ADC with Error Correction
Diff. Nonlinerarity
< 0.5 % RMS, typ. < 1 % peak-peak
Data Acquisition
Histogram Mode
Method
on-board multi-dim. histogramming process
Dead Time
100 ns, independent of computer speed
Saturated Count Rate
10 MHz
Useful Count Rate
5 MHz
Channels / Pixel
4096
1024
256
64
16
4
1
Max. Scanning Area
16 x 16
64 x 64
128 x 128
256 x 256
512 x 512
1024 x 1024
2048 x 2048
Max. Counts / Time Channel
16 bits
Overflow Control
none, stop, repeat and correct
Collection Time
0.1 µs to 100,000 s
Diplay Interval Time
10 ms to 100,000 s
Repeat Time
0.1 µs to 100,000 s
Sequencing Recording
Programmable Hardware Sequencer, unlimited recording by Memory swapping, in curve mode and scan mode
Synchronisation with Scanning
Pixel, Line and Frame from Scanning Device
Input Count Enable Control
1 bit, TTL
Input Experiment Trigger
TTL
Data Acquisition
FIFO / Parameter-Tag Mode
Method
Time and wavelength tagging of individual photons and continuous writing to disk
Online Display
Decay functions, FCS, Cross-FCS, PCH MCS Traces
FCS Calculation
Multi-tau algorithm, online calculation and online fit
Number of counts of decay / waveform recording
unlimited
Dead Time
100 ns
Saturated Count Rate, Peak
10 MHz
Sustained Count Rate (bus transfer limit)
typ. 4 MHz
Output Data Format (ADC / Macrotime / Routing)
12 / 12 / 4
FIFO Buffer Capacity (Photons)
2 * 106
Macro Timer Resolution, Internal Clock
25 ns, 12 bit, overflows marked by MOTF entry in data stream
Input Macro Timer Resolution, Clock from Sync
10 ns to 100 ns, 12 bit, overflow marked by MOTF entry in data stream
Input Curve Control (external Routing)
4 bit, TTL
External Event Markers
4 bit, TTL
Input Count Enable Control
1 bit, TTL
Input Experiment Trigger
TTL
Data Acquisition
FIFO / Parameter-Tag Imaging Mode
Method
Buildup images from time- and wavelength tagged data
Online Display
up to 8 Images in different time and wavelength windows
Synchronisation with Scanner
via Frame Clock, Line Clock and Pixel Clock Pulses
Detector / Wavelength Channels
1 to 16
Image resolution (64-bit SPCM Software)
No. of Time Channels
64
256
1024
4096
No. of Pixels, 1 Detector Channel
4096 x 4096
2048 x 2048
1024 x 1024
512 x 512
No. of Pixels, 16 Detector Channels
1024 x 1024
512 x 512
256 x 256
128 x 128
Operation Environment
PC System
Windows 7 to 10, > 8 GB RAM, 64 bit operating system recommended
PC Interface
PCI
Power Consumption
approx. 12 W from +5 V, 0.7 W from +12 V
Dimensions
240 mm x 130 mm x 15 mm
商品属性 [Saturated Count Rate] 10 MHz [Time Channel Width] Down to 813 fs [Time Resolution ] 2.5 ps RMS (6.6 ps FWHM)