FLIM Upgrades for Zeiss LSM 710 / 780 / 880
OVERVIEW
Two Fully Parallel TCSPC FLIM Channels
Confocal and/or Multiphoton NDD FLIM
Recording by two bh's Multidimensional TCSPC Technique
Compact bh Simple-Tau or Power-Tau System
Two HPM-100-40 GaAsP Hybrid Detectors
Optional: HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM
Optional: HPM-100-50 Hybrid Detectors for NIR FLIM
Optional: Multi-Wavelength FLIM
Excellent Time Resolution: Electrical IRF Width 3.5 ps FWHM
Excellent Timing Stability - No Need to Record IRF
Time Channel Width Down to 405 fs
Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels
Simultaneous FLIM/PLIM
Detection Wavelength Range from 360 nm to 900 nm
Excitation by Ti:Sa Laser, OPO, or by bh ps Diode Lasers
Identical Systems for Multiphoton FLIM and Confocal FLIM
Detectors can be Swapped Between NDD Port and Confocal Port
Systems are Modular: More FLIM Channels Can be Added
Data Analysis by bh SPCImage NG
The FLIM systems for the Zeiss LSM 710 /780 / 880 / 980 family laser scanning microscopes are based on bh’s Multi-Dimensional TCSPC technique and 64 bit Megapixel FLIM technology. The systems feature single-photon sensitivity, excellent spatial and temporal resolution, multi-exponential decay analysis, and short acquisition time. The systems are compatible both with multiphoton excitation and diode laser excitation. The system fully exploits the fast beam scanning capability of the Zeiss LSM. The functions include advanced techniques like multi-wavelength detection, Z stack FLIM, time-series FLIM, spatial and temporal mosaic FLIM, fluorescence lifetime-transient scanning (FLITS), and phosphorescence lifetime imaging (PLIM). Main applications are the measurement of molecular environment parameters, FRET measurements, autofluorescence FLIM of tissue, metabolic imaging, and plant physiology. For details please see 350-page user handbook, 'Modular FLIM systems for Zeiss LSM 710/780/880 Laser Scanning microscopes' and Addendum to Handbook for LSM 980 Microscopes.
FUNCTIONS
Multiphoton FLIM
Confocal FLIM
Parallel Recording in 1 to 4 Wavelength Channels
Multiplexed Recording at two Excitation Wavelengths
Multi-Wavelength FLIM in 16 Wavelength Channels
Multiphoton Multi-Spectral NDD FLIM
Fast Acquisition FLIM by Optional FASTAC FLIM System
Near-Infrared FLIM
Time Series FLIM: Fast time Series or Microscope-Controlled Time Series
Z Stack FLIM
Spatial Mosaic FLIM
Temporal Mosaic FLIM of Physiological Effects, down to 40ms per Image
FLITS: Recording Transient Lifetime Effects in a Line Scan, Resolution down to 1 ms
PLIM (Phosphorescence Lifetime Imaging) Simultaneously with FLIM
Fast Preview Mode
Easy Switching Between Instrument Configurations and Operation Modes
Runs at Any Scan Rate
High Resolution Images, Up to 2048 x 2048 Pixels
FCS and Cross-FCS, No afterpulsing Due to Use of Hybrid Detectors
Selected Specifications
Lifetime measurement: time-domain
Excitation: High-frequency pulsed lasers
Principle: TCSPC FLIM by bh's Multi-dimensional TCSPC technique
Buildup of lifetime images: Distribution of photons over arrival times and scan coordinates
Scan rate: Works at any scan rate
Buildup of fluorescence correlation data: Correlation of absolute photon times
General operation modes: FLIM in two spectral or polarisation channels, Multi-wavelength FLIM
Time-series FLIM, Microscope-controlled time series, Z-Stack FLIM, Mosaic FLIM, x,y, z, temporal,
Excitation-wavelength multiplexed FLIM, FLITS (fluorescence lifetime-transient scanning), PLIM (phosphorescence lifetime imaging) simultaneously with FLIM, FCS, cross FCS, gated FCS, PCH, single-point fluorescence decay recording, single-point phosphorescence decay recordingTCSPC System: bh Simple Tau or Power Tau TCSPC system
Number of parallel TCSPC / FLIM channels: typ. 2, max 4
TCSPC / FLIM modules: SPC-150 NX or SPC-180 NX
Electrical time resolution: 1.5 ps rms / 3.5 ps fwhm
Minimum time channel width: 405 fs
Timing stability over 100 seconds: Better than 0.8 ps rms
Timing stability over 30 minutes: Better than 5 ps rms
Saturated count rate: 10 MHz per channel
Input from detector: constant-fraction discriminator
Reference (SYNC) input from Laser: constant-fraction discriminator
Synchronisation with scanning: via frame clock, line clock and pixel clock pulses
Scan rate: works with any scan rate
Synchronisation with laser multiplexing: via routing function of TCSPC modules
Recording of multi-wavelength data: simultaneous in 16 channels, via routing function
Experiment trigger function: TTL, used for Z stack FLIM and microscope-controlled time series
Operation modes of TCSPC system: Single f(t), oscilloscope, f(txy), f(t,T), f(t) continuous flow
FIFO (correlation / FCS / MCS) mode, Scan Sync In imaging, Scan Sync In with continuous flow
FIFO imaging, FIFO Imaging combined with with MCS imaging, mosaic imaging, time-series imaging
multi-detector operation, laser multiplexing operation, cycle and repeat function, autosave function
Display Functions (online): Intensity images, gated intensity images, lifetime images, decay curves in ROIs, decay curves, FCS curves, intensity traces.
No. of images displayed simultaneously: 8
Max. Image Format, pixelsX x pixels Y x time channels: 4096 x 4096 x 64, 2048 x 2048 x 256, 1024 x 1024 x 1024, 512 x 512 x 4096, 256 x 256 x 4096Software:
Data Acquisition Software: bh SPCM
Operating System: Windows 10 64 bit
Data Analysis Software: bh SPCImage NG
Principle of Data Analysis: MLE with GPU processing
Model functions: Single, double, triple exponential decay, single, double, triple exponential decay with incomplete decay, shifted component model.
IRF modelling: Syntethic IRF function fit to decay dataExcitation Sources, for confocal FLIM: One to four ps diode lasers
Available wavelengths: 375 mm to 785 nm
Repetition rate: 20, 50, 80 MHz and CW
Pulse width: 40 ps to 100 ps
Excitation source, for multiphoton FLIM: Ti:Sa laser of Zeiss LSM NLO systemDetectors: HPM-100-40 hybrid detector with GaAsP cathode, 300 to 710 nm
Optional: HPM-100-06 detector with <20 ps fwhm IRF width, 300 to 600 nm
Optional: HPM-100-50 detector, 400 to 900 nm
Optional: MW-FLIM GaAsP multiwavelength detector
描述 [描述] based on bh’s Multi-Dimensional TCSPC technique and 64 bit Megapixel FLIM technology