MicroMirror TIRF systems
Features
Integrated piezo nanopositioning system
Integrated imaging & motion control platform
Spatially separated excitation & emission beams
Adjustable micromirrors
Open access to optical pathways
Highly stable, yet flexible, platform
Integrated slide holder
TIRF Lock feedback control
Advantages
Superior signal-to-noise ratio
Eliminates dichroic mirrors
Optical pathway accessibility
Facilitates multiple laser lines
Proven design
Enabling technology for studying complex biomolecular interactions
Commercial solution
Product Description
The Mad City Labs' MicroMirror TIRF microscope is part of our RM21™ microscope family and draw on our expertise with high precision, high stability piezo nanopositioning systems and microscopy solutions. The microscopy technique of colocalization single molecule spectroscopy (CoSMoS) is the only proven method to study the ordered assembly and function of multicomponent biomolecular machines. The MicroMirror TIRF microscope is the only commercially available CoSMoS microscope.
The MicroMirror TIRF microscope uses through-theobjective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micro-mirrors positioned at the back aperture of the objective lens. Spatially separating the excitation and emission beams and eliminating the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment. Typical biomolecular systems studied using micro-mirror TIRF incorporate 3 or more laser lines which can be difficult to accomodate in conventional dichroic TIRF instruments. Each micro-mirror is mounted to a multi-axis precision mount allowing the user to make fine adjustments to the optical pathway. As with all RM21™ microscopes it is designed to maximize optical pathway accessibility and flexibility, while also simplifying the optical alignment.
A compelling advantage of our MicroMirror TIRF microscope the open access to the entry and exit optical pathways, integrated XYZ closed loop piezo nanopositioning system. The XYZ closed loop nanopositioner uses proprietary PicoQ® sensors for sub-nanometer precision and high stability. A z-axis micropositioner with integrated sample holder is mounted above the piezo nanopositioning system.
Completing our MicroMirror TIRF microscope is the optional TIRF Lock™ feedback control consisting of a QPD sensor, TIRF Lock controller and software. The TIRF Lock module maintains the TIR signal through software feedback to the piezo nanopositioner. The MicroMirror TIRF microscope is a proven design and offers a simple yet flexible instrument platform for single molecule research.
Technical Specifications - Imaging & Motion Control
Micropositioning motion
XYZ
Ranges of motion (XY)
25 mm
Range of motion (Z)
10 mm
Body Material
Anodized Aluminum
Nanpositioning Motion
XYZ
Ranges of motion (XYZ)
200 μm
Resolution
0.4 nm
Recommended max. load (horizontal)*
0.5 kg
Body Material
Aluminum
Controller†
Nano-Drive®
Digital Interface
USB 2.0
Analog Input
0 to 10V
Synchronization outputs
TTL (4)
Nanopositioner Software†
Nano-Route®3D
TIRF Software compatibility
GLIMPSE
Technical Specifications - MicroMirror Mounts
Micro-Mirror motion
5 axis
Micro-Mirror diameter
3 mm
Mount material
Aluminum
Technical Specifications - TIRF Lock™ Feedback
TIRF-Lock™ Sensor
Quadrant Photodiode
Controller
TIRF Lock
Digital interface
USB 2.0
Software
TIRF Lock
* Larger load requirements should be discussed with our engineering staff.
† See Software Compatibility, above
商品属性 [行程] (XY)25 mm (Z)10 mm [负载] 0.5 kg [分辨率] 0.4 nm