GATTA-STED 3D Nanoruler
Verify the resolution of your 3D STED microscope
3D nanorulers from the GATTA-STED series are the ideal resolution rulers for high-performance 3D STED super-resolution microscopes. The samples consist of two spots oriented in axial direction to study the resolution capabilities in z.
The GATTA-STED 3D 90R Expert Line is a nanoruler with two spots seperated by 90 nm. The spots are marked with multiple ATTO 647N fluorophores, which belong to the best STED dyes available. To stabilize the fluorophores the samples are embedded in ProLong Gold Antifade. The samples can show a distribution of angles with lying, tilted and standing pillars. Most of the rulers are standing (angle > 45°).
A detailled study of the GATTA-STED 3D 90R Expert Line nanorulers can be found in the following publication:
Göttfert, F. et al. Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent. Proc Natl Acad Sci USA (2017). doi:10.1073/pnas.1621495114
GATTA-STED 3D 90R Super-resolution image of a GATTA-PAINT 3D 90R Expert Line nanoruler. Image courtesy of Abberior Instruments GmbH
GATTA-PAINT 3D 90R Expert Line
Color
red
Fluorophore
ATTO 647N
Recommended laser source
630—650 nm
Surface density nanoruler
≈ 1/μm²
Axial distance
90 nm
商品属性 [颜色] red [荧光团] ATTO 647N [表面密度] ≈ 1/μm² [推荐光源] 630—650 nm [距离] 90 nm