Low-Cost TCSPC Module
Time Channel Width Down to 813 fs
Electrical Time Resolution 2.5 ps RMS (6.6 ps FWHM)
10 MHz Saturated Count Rate
Fluorescence Decay Recording
Phosphorescence Decay Recording
Photon Correlation
Single-Molecule Spectroscopy
Free Instrument Software for Windows 7/8/10 (Realtime Calculation and Fitting of FCS Curves)
Parallel Operation of Up to 4 Modules
Available as Multi-Module Package e.g. SPC-132-EMN, SPC-133-EMN and SPC-134-EMN
The SPC-130-EMN is a small size and low-cost time-correlated single photon counting (TCSPC) module (patented) with high-time resolution for standard fluorescence lifetime applications and for applications requiring a large number of parallel TCSPC channels. The board offers a PCI interface and can be easily installed in nearly all PCs.
General Information
The SPC-130-EMN boards have all standard photon distribution modes, the continuous-flow mode, and the FIFO (time-tag) mode implemented. However, this board has no imaging mode. The SPC-130-EMN board can thus be used for traditional fluorescence lifetime experiments, diffuse optical tomography, stopped flow experiments, single molecule detection and combined FCS/lifetime experiments. The SPC-130-EMN has especially overvoltage-hardened signal inputs. They are reliable and rugged, and can be used even under harsh conditions. When ordered in large quantities for OEM applications, the bh SPC-130-EMN modules offer unprecedented price-performance ratio. The module is upgradable to SPC-150N for fluorescence lifetime imaging microscopy (FLIM) and other laser scanning applications.
Measurement Software Included
The SPCM operating and measurement software is included with all SPC series modules. SPCM provides online calculation and display (2D, 3D) of data (decay curves, FCCS) acquired in multiple operation modes. SPCM software undergoes active continuous development. SPCM receives frequent updates with new features and bug fixes.
FLIM and FCS Data Analysis
For advanced fluorescence lifetime single-curve data analysis, please use SPCImage.
For advanced fluorescence correlation spectroscopy (FCS) and cross-correlation (FCCS) data analysis, please use Burst Analyzer.Custom Programming Libraries (DLL, LabVIEW)
For automation and custom software integration, DLLs and LabVIEW drivers are available.
Photon Channel
Principle
Constant Fraction Discriminator (CFD)
Discriminator Input Bandwidth
4 GHz
Time Resolution (FWHM/RMS, electr.)
6.6 ps / 2.5 ps
Variance in Time of IRF max.
< 1 ps over 50 s
Optimum Input Voltage Range
-30 mV to -500 mV
Min. Input Pulse Width
200 ps
Threshold
0 to -250 mV
Zero Cross Adjust
-100 mV to 100 mV
Synchronisation Channel
Principle
Constant Fraction Discriminator (CFD)
Discriminator Input Bandwidth
4 GHz
Optimum Input Voltage Range
-30 mV to -500 mV
Min. Input Pulse Width
200 ps
Threshold
0 to -250 mV
Frequency Range
0 to 150 MHz
Frequency Divider
1, 2, 4
Zero Cross Adjust
-100 mV to 100 mV
Time-to-Amplitude Converters / ADCs
Principle
Ramp Generator / Biased Amplifier
TAC Range
50 ns to 5 µs
Biased Amplifier Gain
1 to 15
Biased AmplifierOffset
0 % to 50 % of TAC Range
Time Range incl. Biased Amplifier
3.3 ns to 5 µs
Min. Time Channel Width
813 fs
ADC Principle
Flash ADC with Error Correction
Diff. Nonlinerarity
< 0.5 % RMS, typ. < 1 % peak-peak
Data Acquisition
Histogram Mode
Method
on-board 2-dim. histogramming process
Online Display
Decay curves (waveforms)
Dead Time
100 ns, independent of computer speed
Saturated Count Rate
10 MHz
Sustained Count Rate
10 MHz
Max. useful Count Rate (50 % loss)
5 MHz
Max. Number of curves in memory
65536
16384
4096
1024
256
64
Number of Time Channels / Curve
4
16
64
256
1024
4096
Max. Counts / Channel
16 bits
Overflow Control
none, stop, repeat and correct
Collection Time
0.1 µs to 100,000 s
Diplay Interval Time
10 ms to 100,000 s
Repeat Time
0.1 µs to 100,000 s
Curve Control (Internal Sequencing)
Programmable Hardware Sequencer
Input Curve Control (Routing)
4 bit, TTL
Input Count Enable Control
1 bit, TTL
Input External Event Markers
4 bit, TTL
Input Experiment Trigger
TTL
Data Acquisition
FIFO / Parameter-Tag Mode
Method
Time and wavelength tagging of individual photons and continuous writing to disk
Online Display
Decay functions, FCS, Cross-FCS, PCH MCS Traces
FCS Calculation
Multi-tau algorithm, online calculation and online fit
Number of counts of decay/ waveform recording
unlimited
Dead Time
100 ns
Saturated Count Rate, Peak
10 MHz
Sustained Count Rate (bus transfer limit)
typ. 4 MHz
Output Data Format (ADC / Macrotime / Routing)
12 / 12 / 4
FIFO Buffer Capacity (Photons)
2 * 106
Macro Timer Resolution, Internal Clock
25 ns, 12 bit, overflows marked by MOTF entry in data stream
Input Macro Timer Resolution, Clock from Sync
10 ns to 100 ns, 12 bit, overflow marked by MOTF entry in data stream
Input Curve Control (external Routing)
4 bit, TTL
Input Count Enable Control
1 bit, TTL
Input Experiment Trigger
TTL
Operation Environment
PC System
Windows 7 to 10, > 8 GB RAM, 64 bit operating system recommended
PC Interface
PCI
Power Consumption
approx. 12 W from +5 V, 0.7 W from +12 V
Dimensions
240 mm x 130 mm x 15 mm
商品属性 [Saturated Count Rate] 10 MHz [Time Channel Width] Down to 813 fs [Time Resolution ] 2.5 ps RMS (6.6 ps FWHM)